TTF-1 and Napsin a Double Staining in Diagnosing Lung Adenocarcinoma

Clinical significance of TTF‐1 protein expression and TTF‐1 gene amplification in lung adenocarcinoma

The majority of lung adenocarcinomas express the lineage-specific thyroid transcription factor-1 (TTF-1). We recently reported that in a subset of lung adenocarcinomas the TTF-1 gene is amplified. Although the prognostic significance of TTF-1 expression has been previously investigated, the significance of TTF-1 amplification has not been established. We studied 89 consecutive patients with lun...

Non-small Cell Lung Carcinomas Lacking Morphologic Differentiation on Biopsy Specimens: Utility of an Immunohistochemical Panel Containing TTF-1, Napsin

TTF-1 [SPT24] Staining Specificity in Lung Adenocarcinoma vs. Lung Squamous Cell Carcinoma is Markedly Improved with Titer Optimization and Cut-Off Values

Materials and Methods Formalin-fixed paraffin-embedded lung SqCC (n=137) and LADC (n=60) tissue microarrays were acquired. DSG3/p40 + Napsin A double stain cocktail (Biocare Medical, Concord, CA), TTF-1 [SPT24] and TTF-1 [8G7G3/1] antibody titers were optimized for IHC. SPT24 was optimally titered at 1:1200. The double stain antibody cocktail was detected by using a polymer double stain detecti...

Napsin A levels in epithelial lining fluid as a diagnostic biomarker of primary lung adenocarcinoma

BACKGROUND It is crucial to develop novel diagnostic approaches for determining if peripheral lung nodules are malignant, as such nodules are frequently detected due to the increased use of chest computed tomography scans. To this end, we evaluated levels of napsin A in epithelial lining fluid (ELF), since napsin A has been reported to be an immunohistochemical biomarker for histological diagno...

The High Diagnostic Accuracy of Combined Test of Thyroid Transcription Factor 1 and Napsin A to Distinguish between Lung Adenocarcinoma and Squamous Cell Carcinoma: A Meta-Analysis

BACKGROUND Accurate classification of non-small cell lung cancer (NSCLC) using morphological features has several limitations. However, the use of thyroid transcription factor 1 (TTF-1) and Napsin A as markers for the identification of various subtypes of NSCLC has shown promise. This meta-analysis was designed to evaluate the diagnostic value of combined TTF-1 and Napsin A test to distinguish ...